WebMay 25, 2024 · Our second application involved a transgenic approach (Tg-CIBOP) by crossing the Rosa26TurboID mouse with Camk2a-Cre Ert2 mice 26, 27 to label Camk2a neurons and with Aldh1l1-Cre Ert2 mice 28,... WebApr 18, 2024 · These findings indicate that Aldh1l1-cre drives AS loss of Kir4.1 function but does not change glutamate uptake/transporter expression. Moreover, we did not observe gliosis or microglial activation at any time points analyzed in …
AAV-Mediated Astrocyte-Specific Gene Expression under Human ALDH1L1 ...
WebDec 11, 2024 · The Aldh1l1 -CreER T2 knock-in mice were identified through PCR analysis (Fig. S1A–C). The sizes of the PCR products obtained from the 5’ homology arm, the 3′ … WebDec 31, 2024 · The h ALDH1L1 ALDH1L1 locus was assessed for genomic conservation using the Vistabrowser2 []. h ALDH1L1 promoter was generated into MluI-BamHI restriction enzyme sites of AAV-MCS (multi cloning site) expression vector (Cell Biolabs, Inc. cat# VPK-410) by in-Fusion cloning scheme as described previously []. AAV-hALDH1L1-Cre … jbl u504
reawards – REJournals
WebApr 13, 2024 · They crossed them to Aldh1l1-Cre/ERT2 BAC transgenic mice. These can be induced to express the Cre recombinase driven by the promoter for astrocyte-specific aldehyde dehydrogenase 1 family member L1. This shuts off ApoE expression in astrocytes ( Srinivasan et al., 2016 ). Tamoxifen added to the drinking water flipped the Cre switch. WebNov 30, 2024 · We found that the GFAP-Cre 12 , hGFAP-CreER 23 , and Aldh1l1-Cre 24 mouse lines, that were reported to produce astrocytespecific gene expression in many other brain regions [23][24][25] ... WebSep 7, 2024 · Recently, Aldh1L1 has been shown to be a pan-astrocyte marker and that its promoter can be used to specifically target astrocytes using the Cre-loxP system. In this … jbl u501