WebApr 6, 2010 · Bovine serum albumin (BSA) is a commonly used model protein in the development of pharmaceutical formulations. In order to assay its release from various … WebSep 5, 2024 · High-performance liquid chromatography (HPLC) is a technique in analytical chemistry used to separate the components in a mixture, and to identify and quantify each component. It was initially discovered as an analytical technique in the early twentieth century and was first used to separate colored compounds.
Pharmaceutical Determination of Gemifloxacin by Using HPLC …
WebNov 9, 2015 · Nano HPLC-MS/MS separation and detection of peptides for proteomic analysis is usually performed upon tryptic digest of proteins and peptide pre-concentration on trap columns. Pre-concentration on trap columns is needed for sample wash (removal of salts and impurities), sample focusing prior to separation, and volume reduction. Usually, … WebJul 28, 2024 · As observed in Figure 2A, most of the saikosaponins could be well separated within 20 min, providing a basis for the accuracy of the HPLC-based quantitative method. The linearity, precision, repeatability, stability, and recovery of the established HPLC-MRM/MS method were evaluated to validate the feasibility of quantitative analysis. translate korean name
A Fast and Accurate UPLC Method for Analysis of
WebJul 31, 2011 · The method showed adequate separation of Gemifloxacin from its stress induced degradation products and excipients with resolution greater than 1.5 within 11 minutes. The method is therefore considered as stability indicating, rapid and suitable for the chromatographic purity and assay determination of Gemifloxacin not only in routine … WebThe method consists of six steps: (1) crude extraction of α-amylase by centrifugation and filtration; (2) α-amylase purification by desalting and anion-exchange chromatography; (3) reaction of the purified amylase with boron-dipyrromethene (BODIPY)-labeled substrate, which releases a fluorescent fragment upon digestion of the substrate, thus … WebMay 23, 2024 · macromolecule, such as BSA, 100 µl at 2 mg/ml) and saved as a new method (see instructions in Data Analysis, Step 3x). 3. Write a MALS method (it's easiest to start from an existing method) a. File → New → Experiment from Method 4. Expand configuration menu 5. Double-click HPLC Device (HPLC Pump) a. Set the flow rate (eg. … translate korean name to tagalog