WebCircular RNA (CircRNA) is yet another vital addition to the noncoding RNA family. They are mainly derived by fusion of downstream 3' splice donor with upstream 5' splice acceptor … WebNov 12, 2024 · Circular RNA is progressively reported to occur in various species including mammals where it is thought to be involved in the post-transcriptional …
A Highly Efficient Strategy for Overexpressing circRNAs
WebOct 14, 2024 · CircRNAs have also been used to evaluate the effectiveness of tumor-treatment methods and supervise tumor stress. For instance, Xie et al. ( 70) … WebJul 6, 2024 · This study pioneers the use of exogenous circRNA for robust and stable protein expression in eukaryotic cells and demonstrates that circRNA is a promising … notebook test chip bestenliste
Influence of circular RNA topology on microRNA stability
WebJul 18, 2024 · Circular RNAs (circRNAs) are stable and prevalent RNAs in eukaryotic cells that arise from back-splicing. Synthetic circRNAs and some endogenous circRNAs can … We first sought to uncover principles behind circRNA vector topology that are necessary for strong translation. We began by synthesizing circRNAs with a coxsackievirus B3 (CVB3) IRES (denoted iCVB3) downstream, or 3′, of the reporter NanoLuc gene, maintaining the reading frame through the … See more Synthesis of circRNAs via intron-assisted splicing and RNaseR digestion has been previously described4, but rapid creation of different circRNA species was difficult. To enable higher-throughput testing of circRNAs, we … See more 5′ and 3′ UTRs in mRNAs can recruit RNA-binding proteins (RBPs) that enable strong translation as well as aspects of post-transcriptional … See more We previously showed that circRNAs can trigger immune responses in vivo that can be avoided by modifying circRNAs with m6A4,11. However, the effect of m6A incorporation on circRNA translation is unknown. To … See more Viral IRESs are diverse and highly structured RNA regions found primarily in viral 5′ UTRs that promote cap-independent translation30,31,32. Because iCVB3, the baseline … See more WebJul 25, 2024 · Here, Chen et al. create a circular RNA (circRNA) modular cloning platform that enables testing of numerous sequence variations and independent optimization of … how to set number format in word